Investigating the Effect of Biogas Slurry on the Morpho-Physiological Traits of Mustard Plant

https://doi.org/10.5281/zenodo.18403038

Abstract

Mustard plant is an herbaceous annual plant belonging to a Brassicaceae family. Salt stress is a major environmental stress that affects plant growth and development. The aim of this research is to investigate the effect of biogas slurry on the morpho-physiological traits of  mustard plant  under salt stress. The experiment consisted of four levels of salinity stress (0mM, 50mM, 100mM, 150mM) and three level of biogas slurry per pot (0 g, 20 g, 40 g) in a completely randomized design with three replicates. An appropriate statistical tool was used to analyze the data by using COSTAT software. The results indicated that morphological parameters decreased with increasing NaCl stress (50mM, 100mM, 150mM) but biogas slurry (20g,40g) mitigated the negative effect. Physiological parameters also decreased with increasing NaCl stress (50mM, 100mM, 150mM) but biogas slurry (20g, 40g) reduced the effect of stress. In case of ion analysis Na⁺ content increased with increasing NaCl stress, biogas slurry reduced Na⁺ uptake in mustard. In case of K⁺ and Ca²⁺ increased with increasing NaCl stress (50mM, 100mM, 150mM), biogas slurry treated plants maintained higher K⁺ and Ca²⁺ level. In case of antioxidant enzyme, increased with increasing NaCl stress, biogas slurry further increased enzyme activity, indicating improved stress tolerance. H₂O₂ and MDA content increased with increasing stress. Biogas slurry reduced H₂O₂ and MDA content under stress, indicating decreased oxidative stress.